中风模型 – MCAO

缺血性中风的动物模型是诱导脑缺血的过程。 目的是研究该疾病的基本过程或潜在的治疗方案，以及拓展病理生理学知识和/或改善人类缺血性中风的医学治疗。

目前已知不同物种中的几种模型会产生脑缺血。完全缺血模型，无论是完整的还是不完整的，往往更容易执行。然而，与局灶性中风模型相比，它们与人类中风的相关性较低，因为全脑缺血不是人类中风的常见特征。然而，在各种情况下，全脑缺血也是相关的，例如，由于心脏骤停导致的全脑缺氧性脑损伤。不同物种对各种类型的缺血性损伤的易感性也各不相同。例如沙鼠。它们没有Willis环，并且可以诱导单独的颈总动脉阻塞诱发中风。
大脑中动脉闭塞（MCAO）是小鼠和大鼠中常用的中风模型。该模型的主要缺点是细丝插入时可能产生不完全闭塞。为了确保模型制作成功，可以监测皮质血流以确保已经诱发了中风。 这可以通过使用传统的激光多普勒灌注监测来完成，通过将探针粘合到颅骨或通过激光散斑对比成像，可以提供脑血流（CBF）的实时图像
推荐产品: Stroke model kit, PSI HR, PF 6000.

PeriCam PSI 血流测量系统

PeriCam PSI系统提供的数据实时显示整个过程中灌注的动态和空间分布。这不仅可以确认完全闭塞，还可以通过量化面积（mm²）来研究中风的程度。

相关文献

1. Ansari, S., Azari, H., McConnel, D.J., Afzal, A., Mocco, J. Intraluminal middle cerebral artery occlusion (MCAO) model for ischemic stroke with laser Doppler flowmetry guidance in mice, Journal of visualized experiments, 2011
2. Ansari, S., Azari, H., McConnel, D.J., Afzal, A., Mocco, J. Intraluminal middle cerebral artery occlusion (MCAO) model for ischemic stroke with laser Doppler flowmetry guidance in mice, Journal of visualized experiments, 2011
3. C‐C Chemokine Receptor Type 5 (CCR5)‐Mediated Docking of Transferred Tregs Protects Against Early Blood‐Brain Barrier Disruption After Stroke. Peiying Li, Long Wang, Yuxi Zhou, Yu Gan, Wen Zhu, Yuguo Xia, Xiaoyan Jiang, Simon Watkins, Alberto Vazquez, Angus W. Thomson, Jun Chen, Weifeng Yu, Xiaoming Hu. 2017, Journal of the American Heart Association, p. e006387.
4. Endothelium-targeted overexpression of heat shock protein 27 ameliorates blood–brain barrier disruption after ischemic brain injury. Yejie Shi, Xiaoyan Jiang, Lili Zhang, Hongjian Pu, Xiaoming Hu, Wenting Zhang, Wei Cai, Yanqin Gao, Rehana K. Leak, Richard F. Keep, Michael V. L. Bennett, and Jun Chen. 2017, PNAS, Proceedings of the National Academy of Sciences, pp. E1243-E1252.
5. Brain ischemic preconditioning protects against ischemic injury and preserves the blood-brain barrier via oxidative signaling and Nrf2 activation. Tuo Yang, Yang Sun, Leilei Mao, Meijuan Zhang, Qianqian Li, Lili Zhang, Yejie Shi, Rehana K. Leak, Jun Chen, Feng Zhang. 2017, Redox Biology, pp. 323-337.
6. Brain-Derived Glia Maturation Factor β Participates in Lung Injury Induced by Acute Cerebral Ischemia by Increasing ROS in Endothelial Cells. Fei-Fei Xu, Zi-Bin Zhang, Yang-Yang Wang & Ting-Hua Wang. 2018, Neuroscience Bulletin, pp. 1077-1090.
7. The microRNA miR-7a-5p Ameliorates Ischemic Brain Damage by Repressing α-Synuclein. Kim T, Mehta SL, Morris-Blanco KC, Chokkalla AK, Chelluboina B, Lopez M, Sullivan R, Kim HT, Cook TD, Kim JY, Kim H, Kim C, Vemuganti R. 2018, Science Signaling, p. eaat4285.
8. Assessing the effects of Ang-(1-7) therapy following transient middle cerebral artery occlusion. M. M. C. Arroja, E. Reid, L. A. Roy, A. V. Vallatos, W. M. Holmes, S. A. Nicklin, L. M. Work & C. McCabe. 2019, Scientific Reports, p. 3154.
9. Modulation of brain cation-Cl− cotransport via the SPAK kinase inhibitor ZT-1a. Jinwei Zhang, Mohammad Iqbal H. Bhuiyan, Ting Zhang, Jason K. Karimy, Zhijuan Wu, Victoria M. Fiesler, Jingfang Zhang, Huachen Huang, Md Nabiul Hasan, Anna E. Skrzypiec, Mariusz Mucha, Daniel Duran, Wei Huang, Robert Pawlak, Lesley M. Foley, T. Kevin Hitc. 2020, Nature Communications.
10. Endothelium-targeted deletion of the miR-15a/16-1 cluster ameliorates blood-brain barrier dysfunction in ischemic stroke. Feifei Ma, Ping Sun, Xuejing Zhang, Milton H. Hamblin, and Ke-Jie Yin. 2020, Science Signaling